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Immunostaining After organotypic culture or cryosection, the cochlear explants or tissue sections were fixed with 4% PFA, permeabilized with 1% TritonX-100 in PBS, and blocked by incubation in PBT-1 solution (0.1% Triton X-100, 8% donkey serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h

Instead, drugs or supplements acting on GABA receptors may alter sleep structure (e.g., less slow-wave or more REM sleep)

While the movement patterns in the Ischemia-Reperfusion group appear limited, the erratic nature of their movement, characterized by a lack of purposeful direction, is more indicative of their impaired condition

For testing, animals were anesthetized with ketamine (80 mg/kg) and xylazine (10 mg/kg) via i.p