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Saitoh T, Satoh H, Nobuhara M, Machii M, Tanaka T, Ohtani H, Saotome M, Urushida T, Katoh H, Hayashi H

Loguercio, C., & Di Pierro, M

For CBB staining, SDS-PAGE gels were stained for 10 min using staining solution (0.1% Coomassie Brilliant Blue G250, 10% acetic acid, 50% methanol, and 40% H 2 O 2 ) and destained with distilled water overnight

Then the total lysates were run on polyacrylamide gels, proteins were blotted on PVDF-membranes and the membranes were blocked in blocking buffer for 1 h