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The effects of bovine necrotic vulvo-vaginitis on reproductive and production performance of Israeli 1st calf heifers

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Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator

It is widely studied for its connection to tissue repair, collagen production, elastin support, and overall cellular renewal