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That is because so much was happening in such a short time

Next, specimens were dehydrated through an ascending ethanol series, starting with 50% ethanol, followed by incubation in 70% ethanol, 1% uranyl acetate (#21447, Polyscience Europe, Heidelberg, Germany), and 1% phosphotungstic acid (#455970, Merck, Darmstadt, Germany) solution overnight at 4 C

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