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The plasmid for the non-tagged SUN2 expression was cloned by cutting the SUN2-CFP plasmid 65 with EcoRI and BamHI, blunting with Phusion DNA Polymerase (New England Biolabs, M0530), and re-ligating the plasmid thus introducing a stop codon in frame with the SUN2 sequence

31 In addition to its vasorelaxing and antiproliferative roles, NO has an important role in antagonizing the effects of AT-II, endothelins and ROS

All findings were reported as efficacy end points, using the data from all the participants who underwent randomization, excluding those who discontinued treatment because of inadvertent enrolment

Microglia in ALS spinal cords exhibited increased expression of ALOX5 and LPCAT3, indicating dysregulated iron metabolism and activation of ferroptosis