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Next, the gels were equilibrated in transfer buffer (25 mM Tris base, 192 mM glycine, and 10% methanol) for 30 min at 4C, after which the proteins were transferred to polyvinylidene fluoride membranes (Bio-Rad Laboratories) at a constant current of 300 mA for 3 h at 4C

J Hepatol 80:251267 da Silva Marques P, da Fonseca-Martins AM, Carneiro MPD, Amorim NRT, de Pao CRR, Canetti C, Diaz BL, de Matos Guedes HL, Bandeira-Melo C (2021) Eosinophils increase macrophage ability to control intracellular Leishmania amazonensis infection via PGD2 paracrine activity in vitro

These studies will further deepen our understanding of gut microbiota-iron metabolism-ferroptosis regulatory network and also offer theoretical guidance and a translational scaffold for the OA precision-targeted therapy

(3) This potential mechanism and further studies are described below