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Cells were cultured in Minimum Essential Medium supplemented with 10% (v/v) fetal bovine serum, 2 mmol/L l-glutamine, 1 mmol/L sodium pyruvate, 10 mmol/L HEPES, and 50 mol/L penicillin/streptomycin, and maintained at 37 C in a sterile humidified atmosphere with 5% CO 2

AtMORF2 also directly interacts with GUN1 to participate in RS 66 and several genome uncoupled ( gun ) mutants are susceptible to pathogens 6

For best results, we typically recommend a series of Neu-Glow treatments, such as weekly or bi-weekly infusions over a few months

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