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In addition, albumin nanoparticles can bypass the hENT1-dependent uptake pathway due to their natural targeting (binding to the tumor microenvironment through SPARC protein) and unique transmembrane mechanisms (such as gp60 receptor mediated transport), directly increasing the concentration of gemcitabine in resistant tumor cells, and have shown advantages in overcoming hENT1-deficient resistance in pancreatic cancer and bladder cancer models (Bartkowski et al., 2024

The reasoning of the use of kidney cells in the experiments is generally attributed to the formation of cysteine-conjugates in liver followed by transport to the kidney (Correia et al

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J., Bachstetter, A