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100 L of native TMV and mutant TMVconcentrations determined by Lowry assay (0.50.015 g/ml)were serially diluted with sample extraction buffer (0.009 M Na 2 SO 4 , 2% (v/v) Polyvinylpyrrolidone (PVP) 40 k, 0.2% (w/v) Powdered egg (chicken) albumin, 0.003 M NaN 3 , 0.2% (v/v) Tween-20 in washing buffer) was then added to each well and incubated at RT for 2 h

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Again, it was also found that year of study (4th and 6th years had more negative attitudes than 5th year)

Tobacco is responsible for nearly 7 million global deaths annually, according to the World Health Organization, which calls the tobacco epidemic one of the biggest public health threats the world has ever faced. About a quarter of the population over the age of 15 consumes tobacco in the Maldives, according to a 2021 survey by the agency