For BSO treatment, cells were GSH starved for 48 h before washing and addition of EV-F3 and further 0.4 mM BSO (Sigma)
Glutathione is synthesized in two sequential enzymatic reactions: glutamate cysteine ligase (GCL) catalyzes the first, rate-limiting step, and glutathione synthetase couples -glutamylcysteine to glycine in the last step [13]
Glutathione is helpful in accelerating the synthesis of Type1 collagen which leads to the maintenance of the elasticity and firmness of the skin
This microalgae, which offers health-supporting effects thanks to the bioactive components it contains, comes to the fore by enriching the content of various food products such as bakery products (pasta, cookies, bread), dairy products (yogurt, ice cream, cheese), and dietary supplements (129)