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Among the 57 metabolites, the content of 38 metabolites was significantly different between 5-oxp-treated and untreated plants under non-stress or heat stress conditions, and these 38 metabolites were mainly enriched in the pathways of (1) aspartate and glutamate metabolism, (2) nitrogen metabolism, (3) glutathione metabolism, (4) citrate cycle, (5) valine, leucine, and isoleucine biosynthesis, as well as (6) starch and sucrose metabolism (Fig

Cells were fixed with 2% paraformaldehyde (15 min), permeabilized with 0.1% Triton X-100 (10 min) and blocked with 2% BSA (1 h)