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In a two-step procedure, 2-hydroxymalondialdehyde is firstly condensed with p -aminobenzoyl-L-glutamic acid, forming a diimine, which subsequently reacts with triamino-pyrimidinone to obtain folic acid

Dissolve in sterile buffer solution with pH 5~7 and store at -20

Other next-generation agents, such as IPN60090 (IACS-6274), have demonstrated early signs of clinical activity and effective GLS1 inhibition in Phase I studies, with ongoing trials exploring combinations (e.g., with bevacizumab and paclitaxel)

160 In another study, AXIN-2 shRNA lentivirus particles were stereotaxically injected into the substantia nigra pars compacta (SNpc) of parkinsonian rats