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HRT serves as a method for symptom management and a long-term health strategy following ovarian injury (Cattoni et al

The REFUEL study looked at the effect of increasing energy intake in athletic women who either had irregular/infrequent menstrual cycles (oligomenorrhea) or amenorrhea

Then, the cells were washed and suspended in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Sigma-Aldrich) supplemented with 2 g/L sodium bicarbonate, 10% fetal bovine serum (FBS, Sigma-Aldrich), 2% glutamine, and 100 U/mL penicillin-0.1 mg/mL streptomycin (Sigma-Aldrich) and incubated in vitro with RPMI medium (control), tert -Butyl hydroperoxide (tBHP: 200 M) or tBHP plus Ab (22.5 mg/mL) for 30 min at 37C

Acknowledgements This work was created as part of NCCR Catalysis, a National Centre of Competence in Research funded by the Swiss National Science Foundation (grant number 180544 to R.M.B) and by AEI (Spain) (grant number RTI2018-099592-B-C22 to G.J.-O)