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Even though Coomassie Blue staining showed heterogeneity in protein size, incubation with peptide-N4-( N -acetyl-beta-glucosaminyl) asparagine amidase (PNGaseF) , a peptide-endoglycanase that removes high mannose, complex and hybrid N -glycans from proteins, homogenized all isoforms to a sharper band of 2526 kDa, compatible with the predicted molecular weights (MW) of deglycosylated RBD (26.5 kDa, Fig

Oxidative stress from excessive ROS generation has been implicated in pathogenesis of obesity [265]