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Efectos de la administracin de L-carnitina en los niveles de hormonas sexuales y espermatozoides en un modelo de lesin del sistema reproductor de ratas Wistar macho en un entorno hipxico e hipobrico de gran altitud

hTERT RPE-1 cells were cultured in DMEM/F-12 (containing L-glutamine, HEPES buffer and sodium bicarbonate, Sigma-Aldrich) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 100 U ml 1 penicillin (Thermo Fisher Scientific), 100 g ml 1 streptomycin (Thermo Fisher Scientific) and 0.1 mg ml 1 hygromycin B (Roth)

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The results suggested that the mitochondria in the blank group were morphologically more intact, with a rod-like morphology, surrounded by a double-layered membrane, with the inner membrane protruding inwards to form flat plate-like cristae, and that the cristae of the mitochondria were continuous