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Nevertheless, targeting the Wnt pathway alone presents significant challenges for cancer therapy, including poor drug response and toxic side effects (100)

In addition, during liver zonation, knocking down LRP5 and LRP6 causes defective liver zonation in mice during liver regeneration

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The TNF- levels were determined using the rat TNF- ELISA kit from Boster Biological Technology (CA, United States), while IL-1 and IL-6 levels were measured using the rat IL-1 beta/IL-1F2 Quantikine ELISA Kit and the rat IL-6 Quantikine ELISA Kit from R&D Systems (MN, United States), respectively