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Results The p53 TAD2 region plays a crucial role in the interaction with FOXO4 FHD Interactions between FOXO4 and p53 in the nucleus have been reported to promote cellular senescence 34

Under a non-monotonic curve, an increase producing less effect is ambiguous: it cannot distinguish being below the peak from having passed it

Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

That asymmetry has made BPC-157 unusual in the peptide landscape: widely used, extensively studied preclinically, yet almost entirely untested under controlled human conditions