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Samples (30 g protein) were resolved by 420% SDS-PAGE (Biofuraw Precast Gel, Tanon Science, Shanghai, China), transferred to polyvinylidene difluoride membranes, and incubated with one of the following antibodies: LC3 (88588s, Cell Signaling Technology, 1:1000), GPX4 (ab125066, Abcam, 1:1000), p16 (1003-1-AP, Proteintech, 1:1000)

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Pickarts work, researchers looking to test GHK-Cu in a wound healing context should administer a total GHK-Cu dose of 100200 mg to produce therapeutic actions, though the necessary effective dosage may be even smaller [5]

doi: 10.1128/JVI.01347-17 166 AlirezaeiMFlynnCTWoodMRWhittonJL