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Rat iNOS and eNOS conditions After initial denaturation at 95C for 2 min, 2535 cycles of amplifications at 94C 30 sec, 60C for 30 sec and 72C for 45 sec were carried out using Perkin-Elmer 2400 thermal cycler

In each of those column 3 chromatograms, a peak for the confirmation ion was present within 1.4 s of the expected retention time for 2MN and with the proper relative intensities for m / z = 147, 175, and 176 amu

Sivitz WI, Fink BD, Donohoue PA (1999)

He took the blame for the Packers poor special teams effort, particularly one play where they were missing a man on the field during San Francisco's game-winning field goal