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Ferroptotic tubular cells release DAMPs (e.g., HMGB1) and lipid peroxidation products (e.g., 4-HNE), which activate pattern recognition receptors on macrophages and neutrophils, inducing the secretion of pro-inflammatory cytokines (e.g., IL-33 and MCP-1)

Introduction Drugs administered by the subcutaneous route are deposited into subcutaneous tissue (Fig 1)

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The samples were then centrifuged at 18 000 g at 4 C for 15 min, and 20 l of the resulting supernatants was injected into the HPLC column for metabolite quantification, while the pellet was used for protein analysis using the BCA protein assay