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The remaining 44,072 probes were mapped to 20,017 genes with unique Entrez IDs using the collapseRows-function in R-package WGCNA v1.64.1 46 as follows: (i) if there is one probe, that one probe is chosen, (ii) if there are two probes, the one with maximum variance across all samples is chosen (method = maxRowVariance), (iii) if there are more than two probes, the probe with the highest connectivity (summed adjacency) is chosen (connectivityBasedCollapsing = TRUE)
Twice-daily dosing has been used in some acute-phase protocols where intensive early exposure is the design goal
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Conversely, a cohort study using standard dietetic advice to produce intentional weight loss in older adults with T2D demonstrated that every 1 kg of weight loss was an associated 34 months survival prolongation [64], and a meta-analysis of RCTs assessing the impact of intentional weight loss on mortality found a 15%, although the mean age of participants was 52 years [65]
