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delta-Opioid receptor activation attenuates the oligomer formation induced by hypoxia and/or alpha-synuclein overexpression/mutation through dual signaling pathways

In the multicenter PIVENS ( PI oglitazone versus V itamin E versus placebo for the treatment of N onalcoholic S teatohepatitis) trial, 247 subjects with MASH but not diabetes mellitus were randomized to receive 30 mg/day of pioglitazone (an insulin-sensitizing drug), 800 IU/day (536 mg/day) of RRR --tocopherol, or a placebo for 96 weeks (97)

The pellets were resuspended in RIPA buffer and centrifuged at 14,000 g to acquire nuclear extracts