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House dust mites, extracts from the allergenic fungus Alternaria Alternata and cigarette smoke were shown to induce greater barrier dysfunction in air-liquid-interface (ALI) cultures of asthmatic airway epithelial cells compared to those of healthy subjects (68, 83, 84)

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Using less harmful alternatives avoids harmful combustion products, such as tar and other dangerous substances found in tobacco smoke, and the level of carbon monoxide is reduced by up to 98 percent