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The samples were blocked in 3% BSA for 30 min at room temperature, followed by incubating with primary and secondary antibodies
Patients with diabetic kidney disease (DKD) exhibit pronounced gut microbiota dysbiosis, characterized by decreased abundance of commensal bacteria and increased prevalence of uremic toxin-producing bacteria (98) demonstrated that Roseburia intestinalis was significantly decreased while Bacteroides stercoris was markedly increased in the gut of biopsy-proven diabetic nephropathy patients, and this dysbiosis was closely correlated with parameters of lipid metabolism, glucose metabolism, and renal function
105,265,302 Elevated serum uric acid levels heighten the risk of cardiovascular disease (CVD) mortality, potentially due to mechanisms by which hyperuricemia activates the renin-angiotensin system and induces hypertension