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The lower K D value determined from competition with His might arise due to the omitting of the formation of a putative ternary complex between HSA, Cu(II) ion and His in the binding scheme, which should result in higher affinity estimates, since the copper in the ternary complex is found in the HMW peak

Additionally, the pocket normally occupied by the conserved phenylalanine residue of the TIN2 F-X-L-X-P motif was fully obstructed by a crystal contact with a TRF1 TRFH copy in an adjacent asymmetric unit, further limiting the possibility of obtaining XChem hits outside of the leucine pocket

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Assessing the Preparedness of the U.S