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After TMB substrate solution is added, only those wells that contain Human LIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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Colorectal cancer metastatic dMMR immunotherapy (COMMIT) study: A randomized phase III study of atezolizumab (atezo) monotherapy versus mFOLFOX6/bevacizumab/atezo in the first-line treatment of patients with deficient DNA mismatch repair (dMMR) or microsatellite instability-high (MSI-H) metastatic colorectal cancer 9NRG-GI004/SWOG-S1610)

As primarily secreted proteins, IGFBPs are released into the extracellular space, where they interact with IGF-1 (Oxvig and Conover, 2023)