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Following this, the medium was substituted with 200 L of DMSO to dissolve the formazan crystals, and the quantification of viable cells was conducted by measuring the absorbance at 570 nm (Abs) using a spectrophotometer

Histone lactylation mediated by Fam172a in POMC neurons regulates energy balance

The hAMY 3 R cells were cultured in growth medium consisting of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.5 mg/mL Geneticin G418 (Gibco, #10131-027), 0.4 mg/mL Hygromycin (Invitrogen, #10687010) and 250 nM Methotrexate (Sigma, A6770)

Consequently, high levels of cystine build up in their urine where it crystallizes and forms stones that block the kidneys and bladder