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Regarding prescribing: under the Human Medicines Regulations 2012 (regulation 167), doctors and some independent prescribers may prescribe unlicensed medicines (known as 'specials') when clinically necessary and when no licensed alternative is suitable, taking personal responsibility for that decision (GMC: Good practice in prescribing and managing medicines and devices)

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Gradient elution starts from liquid A accounting for 100% of the mixture, during which the proportion of liquid B in the mixture gradually increases until it accounts for 100%, the flow rate is 10ml/min, and the detection wavelength is 215nm

To truly understand how glutathione operates, one must look at the continuous biochemical loop known as the redox (reduction-oxidation) cycle