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After transfer, membranes were blocked in 1% bovine serum albumin in PBS with 0.1% Tween-20 and probed with anti-PARP (NB100-56599, Novus Biologicals, Littleton, CO), anti-Glutathione (AB19534, Abcam, Cambridge, MA), anti-phospho-IB- (9246), anti-IB- (9242), anti-phospho-IKK- (2594), anti-IKK- (2370), or anti-p65 (8242) (Cell Signaling Technology, Danvers, MA) antibodies

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The GRCm38 GENCODE vM23 genome and the corresponding rat genome were used for alignment of the mouse and rat data

59,60,366,367,368 According to previous studies by our group, in the absence of SERT, SSRIs such as fluoxetine can act as direct agonists of astrocytic 5-HT 2B receptors to exert antidepressant-like effects