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Glutathione synthesis is ATP-consuming and depends on the availability of the amino acids cysteine, glutamine and glycine and requires two subsequent reactions, in which -glutamylcysteine synthetase (-ECS or GSH1, EC 6.3.2.2) first catalyzes the formation of -glutamylcysteine (-EC) from cysteine and glutamine and glutathione synthetase (GSHS or GSHS2, EC 6.3.2.3), then catalyzes the formation of glutathione from -EC and glycine

"Glutathione IV infusions can deliver doses up to 10 times higher than oral supplements, ensuring 100% absorption," according to recent research

The formation of gamma-glutamylcysteine is the rate-limiting reaction in GSH synthesis and is feedback inhibited by GSH itself, a mechanism responsible for the regulation of cellular GSH concentration

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