To account for both hypertrophy of individual cells as well as possible increases in cell number, the percent areas of Iba-1 and GFAP staining as proportions of the annotated prefrontal cortex and cerebellum regions were quantified as previously published, 74 the Area quantification FL v2.3.4 module was used
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Physician Richard Carr similarly explains that people who are hot and dry (whom he says are of a bilious or saline constitution) who are generally thirsty, lean, hot and have thin and yellow urine, "unless you would [want to] aggravate them, forbid the Use of Smoaking." 20 Hammond also discusses problems with tobacco use of people of a melancholy temperament, because use of the plant "is a great encreaser of melancholy [black bile] in us, and thereby disposeth our bodies to all melancholy impressions and effects proceeding of that humor." 21 He provides a very detailed explanation of why this is so which basically says that since tobacco purges phlegm - a 'thin' humor - using tobacco means there is less of it is available for making up the bloud resulting in the blood being composed of the thicker 'dregs' or melancholy humors
