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a Transformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid
an enzyme derived from Serratia marcescens
CircLigase enzyme also has activity in ligating a single-stranded nucleic acid having a 3´-hydroxyl ribonucleotide and a 5´-phosphorylated ribonucleotide or deoxyribonucleotide
CircLigase II enzyme also has activity in ligating a single-stranded nucleic acid having a 3´-hydroxyl ribonucleotide and a 5´-phosphorylated ribonucleotide or deoxyribonucleotide
Seamless cloning Master Mix - 20 Rxn size:20 Rxn a Transformation success corresponds toThe Seamless Cloning Master Mix is a ready to use reaction mix for seamless cloning reactions. It allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. It has been rapidly adopted by the synthetic biology community due to its ease of use, flexibility and suitability for large DNA constructs. The Seamless Cloning Master Mix efficiently joins multiple overlapping DNA fragments in a single tube
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