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doi: 10.1039/C9FO02089F 21 CalabreseE

Equal amounts of protein were loaded in 10% sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) and were then transferred from the gel to polyvinylidene fluoride membranes (Millipore, USA)

This vaccine requires only two doses in the first year, followed by one annual booster each year, and it is safe to be used in any stage of bovine pregnancy (98)

Methods for the analysis of vitamin B 12 in food Several methods have been used to determine the vitamin B 12 content in foods including microbiological assays, chemiluminescence assays, polarographic, spectrophotometric, and high-performance liquid chromatography processes