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In general, there is an age-related increase of 5hmC levels in the brain, pointing towards a role for 5hmc in the development of AD [136, 149, 150]

RNA extraction and cDNA synthesis The total RNA was extracted using the MagMAX-96 Total RNA Isolation Kit (Thermo Fisher Scientific) or using the RNeasy Plus Mini Kit (QIAGEN, 74136) following manufacturers instructions

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