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These are most pronounced during dose escalation and typically improve at stable doses, but they are the primary reason patients discontinue treatment

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Walking Recovery Index (WRI) As described previously [13], to objectively measure gait recovery, recordings were carried out using a fixed digital camera placed under the animals (Olympus mju 300 digital, Olympus Optical Co., Ltd., Hachioji, Tokyo, Japan), located 25 cm below and 12 cm to the side of a 100 cm long closed transparent plexiglass tunnel, and measurements and the gait recovery index were calculated, expressed as a coefficient of the length of the footprints with which the animal rests on the ground in certain phases of walking, according to the following Equation (1): where NPL stands for the non-injured animal footprint length, while EPL stands for the experimental animal footprint length, expressed in millimeters

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