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For IMR-5/75, the culture media was supplemented with 5 g/mL Blasticidin (Bio-Sciences) and 50 g/mL Zeocin (Thermo Scientific) for plasmid selection

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Conclusion Whilst severe B12 deficiency has a clear clinical picture with characteristic haematological and/or neurological features, establishing mild or borderline deficiency can be problematic as serum levels are maintained at the expense of tissue concentrations, and therefore serum levels of B12 may be in the low normal or borderline range despite a deficiency

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