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After de-paraffin Cartilage specimens and Cells (in vitro) were washed in PBS and for deactivating endogenous peroxidase action by the use of 3% Hydrogen peroxide (H 2 O 2 ) for 20 min at room temperature with shaking

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Similarly, ovarian oxidative stress model mice exhibit inhibition of the Nrf2/HO-1/GPX4 pathway (Niu et al., 2023), suggesting that GPX4 deficiency-induced ferroptosis may contribute to reduced ovarian reserve

But this question the exact way that I wrote it here was the way it was presented to me by a very scared PA patient who just got diagnosed and whose rheumatologist is telling her very wrong misinformation