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This is the longest continuous biomolecular record of ancient tobacco smoking from a single region anywhere in the world initially during an era of pithouse development, through the late pre-contact equestrian era, and into the historic period, the research team, led by WSU anthropologist Shannon Tushingham, reports in the Proceedings of the National Academy of Sciences

OLADO (UniProt Accession: A0A1S3ZKS9_TOBAC) was expressed using the pET28a(+) vector as an N-terminal His-tagged protein using E