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J Biol Chem 279:31513159 Jeong W, Yoon HW, Lee S-R, Rhee SG (2004b) Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa

Jean-Marc Fritschy for the generous gift of his anti- GABA A R subunit antibodies and Dr Werner Sieghart for his equally generous gift of anti-GABA A receptor 3 subunit antibody

RP-HPLC Used to determine: Chemical purity Degradation products Relative stability over time LC-MS Used to identify: Molecular weight changes Oxidation Deamidation Fragmentation UV-Visible Spectrophotometry Useful for detecting: Oxidation Color changes Chromophore alterations Dynamic Light Scattering (DLS) Used to measure: Aggregation Particle size Solution homogeneity Myth vs

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