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For sub-cultivation and seeding, cells were washed with PBS, detached with 0.25% trypsin/EDTA (Sigma-Aldrich) and resuspended in fresh DMEM/FBS

When reconstituted with bacteriostatic water and stored at 2-8 degrees Celsius, retatrutide remains usable for approximately 30 days

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Then, the virus was injected into the brain region specifically at a speed of 30 nL/min by using a 10 L microsyringe (Gaoge) assembled with a calibrated glass microelectrode (1B 100-3, WPI, USA)