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Materials and methods Cell culture and treatments Human 21NT breast cancer cells were cultured in 1 Modified Eagles medium alpha (alpha MEM) (Gibco), 2.8 M hydrocortisone, 1 g/ml insulin, 10% foetal calf serum (FCS), 1% gluta- max, 10 mM HEPES, 0.1 mM NEAA and 12.5 ng/ml Epithelial Growth Factor (EGF)
In skin care, GHK-Cu is used because it is involved in: Wound healing Collagen and elastin synthesis Skin remodeling and repair As we age, natural levels of GHK-Cu decline
It is a tripeptide composed of three amino acids: cysteine, glutamine, and glycine
350 Additionally, molecular studies on lactylation should concentrate on investigating its specific effects on protein structure and the precise molecular mechanisms through which these effects are mediated, which will contribute to clarifying the orchestration of lactylation on protein function and its subsequent biological significance
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