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OsDHAR was purified by Ni-NTA affinity chromatography [5 mL HisTrap FF column (GE Healthcare, Little Chalfont, UK) on an AKTA FPLC system] and eluted with a step gradient of 250 mM imidazole

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were able to identify specific variants which were only present in the CNS compartment

The first two groups were prepared with unilateral 6-OHDA lesions and members of the first group was treated with MM-201 in DMSO 1001:8 8ml (0.5 mg/kg, i.p.) Members of Group 2 were treated with sterile DMSO 100 :PBS 8ml equivalent volumes to the weights