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Real-time qPCR Total RNA was isolated with TRIZOL reagent (Invitrogen) and reverse transcription was performed by ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO), according to the manufacturers protocol

Conclusion In conclusion, bacteriostatic water is a safe and effective means of reconstituting peptides for use in research and other medical applications

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Naidu et al., 2023), and due to its selective antimicrobial effects, favors the predominance of beneficial microbes (Pino et al., 2017)