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Insert the needle at a 45-degree angle for subcutaneous delivery or 90 degrees for intramuscular delivery

HEK-293T were from American Type Culture Collection (ATCC), and cultured in Dulbeccos modified Eagles medium (DMEM) (Cytiva, SH30003.02) supplemented with 10% fetal bovine serum (FBS, Cytiva)

Studies have shown faster recovery from Achilles tendon transection, improved healing after muscle tears, and even some evidence of gut mucosal repair in models of inflammatory bowel disease

UMP, much like IMP, serves as the intermediate to pyrimidine synthesis and can undergo sequential phosphorylation to form UTP, which can be converted to cytidine (CTP)