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Step 1 1) Glucuronidation: The drug metabolizing enzyme responsible for glucuronidation is UDP-glucuronosyltransferase (UGT)

Paracellular intestinal permeability can be assessed in vivo by using different molecules (e.g., sugars, polyethylene glycols, 51 Cr-EDTA) and ex vivo in Ussing chambers combining electrophysiology and probes of different molecular sizes

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These results indicated that a daily intake of 35 g/kg body weight of BDF could be an effective intervention for human constipation, although further clinical trials are needed