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This small size and simple structure make GHK-Cu relatively straightforward to synthesize at high purity, but its copper-binding chemistry does introduce handling considerations that don't apply to unbound peptides

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To investigate the mechanism of GSH dependent transport mediated by MRP1, we localized on the 3D structure of the transporter 40 two putative GSH binding sites deduced from the numerous mutagenesis studies done in the past two decades on MRP1 compiled in the database 50 , one located in the cytoplasmic face and the other located in the membrane (Fig