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The MCCC2 gene is located on chromosome 5q13.2 and is composed of 19 exons that generate two alternatively spliced mRNAs that encode precursor proteins of 563 and 525 amino acids

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After 24 h, migrated cells on the upper surface of the filter were removed with a cotton swab, while the cells on the bottom of the filter were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with 3% crystal violet solution for 15 min