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While our qPCR analysis relied on a single reference gene (GAPDH) that showed stable expression, future studies could benefit from incorporating multiple reference genes as recommended by the MIQE guidelines [51]
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Thyroid dysfunction in relation to immune profile, disease status, and outcome in 191 patients with COVID-19
Figure 1 In addition, to fully determine the impact of LA on renal dysfunction induced by FA, the direct evaluation of renal function was determined with the indicators as displayed in Supplementary Table 1, FA injection markedly decreased urine volume, glomerular filtration rate (GFR), and filtration fraction compared with control mice, but these indexes could be reversed partially by LA supplementation, without obvious difference between the two dose groups
