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RNase protection assays to detect single-basepair mismatches in RNA:RNA and RNA:DNA hybrids
5 mM MnCl2
leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA
Create modified "RNA" transcripts that are resistant to RNase A-type RNases
QuickExtract-DNA Extraction Solution 1.0, 50 mL size:EACH RNase protection assays to detectSimple, rapid extraction of PCR ready DNA for screening and genotyping type applications Fast: 8 minute extraction protocol for most sample types Simple: No centrifugation steps of spin columns used to help increase yields Automation friendly: Simple protocol integrated easily into automated workflows Safe: Uses only non toxic reagents Recommended for rapid, easy sample prep for T7E1 CRISPR mutation detection assays The QuickExtract DNA Extraction
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