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HUVECs and NIH 3T3 cells in Roswell Park Memorial Institute (RPMI) 1640 and HaCaT in Dulbeccos Minimum Essential Medium (DMEM)/F-12 medium were cultured in the indicated media supplemented with 10% fetal bovine serum (FBS) and maintained at 37C in a humidified environment with 5% CO 2

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Stability and Distribution The molecular architecture enables BPC-157 to remain stable in human gastric juice for over 24 hours and in urine for 4 days, distinguishing it from typical peptides that rapidly degrade